TRT Monitoring

The essential panel for anyone on testosterone replacement therapy. Covers the markers most likely to shift on TRT: haematology (polycythemia risk), hormones (dialling in your dose), and cardiovascular markers.

When to Test

At trough (the morning before your next injection). Fasted blood draw before 10am for accurate hormone levels.

Testing Frequency

Every 3 months for the first year, then every 6 months once stable.

Markers in This Panel(10)

Testosterone

Hormones

Confirms your dose produces therapeutic levels (typically 600-1000 ng/dL trough).

Ref: 8 – 30 nmol/L

Free Testosterone

Hormones

The bioactive fraction; more clinically relevant than total testosterone.

Ref: 200 – 600 pmol/L

Estradiol

Hormones

Aromatisation from testosterone; guides AI need. Target sensitive assay.

Ref: 40 – 160 pmol/L

Haematocrit

Haematology

The primary safety marker on TRT. Levels above 54% increase clot risk.

Ref: 0.38 – 0.5 L/L

Haemoglobin

Haematology

Paired with haematocrit; rising haemoglobin signals polycythemia.

Ref: 130 – 170 g/L

PSA

Hormones

Monitor prostate health; rapid PSA velocity warrants investigation.

Ref: 0 – 4 ug/L

HDL

Lipids

Testosterone can lower HDL; track for cardiovascular risk.

Ref: 1 – 999 mmol/L

LDL

Lipids

Monitor alongside HDL for overall lipid health.

Ref: 0 – 3.4 mmol/L

SHBG

Hormones

Affects free testosterone levels; injection frequency can modify SHBG.

Ref: 10 – 70 nmol/L

ALT

Liver Function

General liver health check; TRT alone rarely elevates, but catches issues early.

Ref: 0 – 40 U/L

Related Compounds

Testosterone Enanthate Testosterone Cypionate Sustanon 250 Anastrozole HCG

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